discussion
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Please No plagiarism, No quotation, no quotes, all your own words. no citing! going through turnitin.com
1.State when the Drosophila A/P and D/V axis is established (1-2 sentences)
2.In addition from question 1, state what specific signaling pathway is involved in priming the D/V axis, and what cells are involved in this process? (5 sentences)
3.There is a hierarchy to forming the body plan of an animal. The one animal we discussed in depth was the developing drosophila embryo. Please explain why defects in GAP genes are by far more deleterious than the loss of Hox/homeobox genes. Keep your response brief.
4.The 3’ UTR is responsible for giving instructions to allocate mRNAs to the right position in a cell. Scientists have been on the lookout for a natural occurring substance encoded in the human genome (a gene) that enhances intelligence in human beings, known as an endogenous nootropic. You were the scientist that found this gene, but it never gets translated into protein. Albeit, you want to investigate where the mRNA strand is allocated in a cell in hopes it may begin to inform you on the function. You generate a floxed alleled mouse where this gene is now primed to be knocked out, and future experiments will be compared to WT mice from the same litter.
If you want to know where the mRNA is positioned in a cell, what experiment would you conduct to investigate where the mRNA is allocated in the cell?
What if you decided to test the 3’ UTR of the “Genius gene” mRNA. What experiment would you do with the 3’ UTR of that gene and GFP tag that can tell you about where this 3’UTR allocates mRNAs?
If you wanted to understand the function of the “Genius gene” what kind of an experiment would you do with the floxed alleled mouse? Explain your two outcomes.
What other experiment would you conduct in a mouse to show that the “Genius gene” has the potential to increase intelligence? Please state what two outcomes you predict would occur after conducting this experiment.
5. The Drosophila embryo has a genetic mutation in the gene twist. Twist is responsible to initiate mesoderm formation in the developing drosophila embryo. Please describe the signaling pathway that leads to twistexpression and how loss of Twist protein may or may not affect germ layer formation.
6.What is the overarching principle of developmental biology that leads to changes in cell shape, identity, and contributes to the diversity in an organism. Clue: it involves genes.

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